翻訳と辞書 Words near each other ・ Bactridium ・ Bactrini ・ Bactriola ・ Bactris ・ Bactris campestris ・ Bactris coloniata ・ Bactris constanciae ・ Bacterial Blight of Soybean ・ Bacterial capsule ・ Bacterial cell structure ・ Bacterial cellular morphologies ・ Bacterial cellulose ・ Bacterial circadian rhythms ・ Bacterial cold water disease ・ Bacterial conjugation ・ Bacterial display ・ Bacterial DNA binding protein ・ Bacterial effector protein ・ Bacterial Filtration Efficiency ・ Bacterial fruit blotch ・ Bacterial genetics ・ Bacterial genome size ・ Bacterial gliding ・ Bacterial glutathione transferase ・ Bacterial growth ・ Bacterial ice-nucleation proteins ・ Bacterial inhibition assay ・ Bacterial kidney disease ・ Bacterial lawn ・ Bacterial leaf scorch Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Bacterial display ： ウィキペディア英語版 Bacterial display Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro protein evolution. Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection procedures (biopanning). This protein engineering technique allows us to link the function of a protein with the gene that encodes it. Bacterial display can be used to find target proteins with desired properties and can be used to make affinity ligands which are cell-specific. This system can be used in many applications including the creation of novel vaccines, the identification of enzyme substrates and finding the affinity of a ligand for its target protein. Bacterial display is often coupled with magnetic-activated cell sorting (MACS) or fluorescence-activated cell sorting (FACS) techniques. Competing methods for protein evolution ''in vitro'' are phage display, ribosome display, yeast display, and mRNA display. Bacteriophage display is the most common type of display system used although bacterial display is becoming increasingly popular as technical challenges are overcome. Bacterial display combined with FACS also has the advantage that it is a real-time technique. ==History== Cell display systems were first used in 1985, when peptides were genetically fused with proteins displayed on the M13 bacteriophage. Bacteriophage display is a commonly used cell display system, although it carries limitations in the size of proteins that can be displayed. Bacterial display was then introduced in 1986, allowing the surface display of larger proteins. Bacterial display systems were first introduced by Freudl et al. and Charbit et al. in 1986, when they used bacterial surface proteins OmpA and LamB to display peptides. Freudl et al. fused peptides with linkers with the ''ompA'' gene, causing the peptides to be expressed in the OmpA proteins. They showed that the proteins were now subject to cleavage by proteinase K. The non-OmpA peptides inserted were therefore a target of proteinase K. Insertion of the foreign peptides did not affect bacterial cell growth. This was the first evidence of using bacterial surface display techniques to express proteins on the surface of cells, without altering the function of the cell. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Bacterial display」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.